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steady-glo lysis/luciferase substrate reagent solution  (Promega)

 
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    Structured Review

    Promega steady-glo lysis/luciferase substrate reagent solution
    Steady Glo Lysis/Luciferase Substrate Reagent Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luciferase+substrate+steady+glo+reagent/steady+glo+substrate/pmc06296672-305-6-11
    Average 90 stars, based on 1 article reviews
    steady-glo lysis/luciferase substrate reagent solution - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: A 27-bp region of the inducible nitric oxide synthase promoter regulates expression in glial cells.
    Article Snippet: .. An equal volume of luciferase substrate (Steady Glo reagent, Promega) was added to all samples, and the luminescence measured in a microplate luminometer (Rosys-Anthos, Anthos, Durham, NC, USA). ..

    Article Title: The novel HSP90 inhibitor, PU-H71, suppresses glial cell activation but weakly affects clinical signs of EAE
    Article Snippet: .. A volume of luciferase substrate (20 μL) (Steady Glo reagent, Promega) was added to all samples, and the luminescence was measured in a microplate reader (VICTOR X4, PerkinElmer). ..

    Article Title: The Anti-Inflammatory Effects of Dimethyl Fumarate in Astrocytes Involve Glutathione and Haem Oxygenase-1
    Article Snippet: .. An equal volume of luciferase substrate (Steady Glo reagent, Promega) was added to all samples, and the luminescence measured in a microplate luminometer (Rosys-Anthos, Hombrechtikon, Switzerland). .. Total GSH levels (GSH+GSSG) were measured in whole cell lysates using a commercial glutathione assay kit (Sigma CS0260).

    Article Title: The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes
    Article Snippet: .. A volume of luciferase substrate (20 μl) (Steady Glo reagent, Promega) was added to all samples, and the luminescence was measured in a microplate luminometer (Rosys-Anthos, Austria). ..

    Article Title: Lymphotoxin beta receptor induces interleukin 8 gene expression via NF-kappaB and AP-1 activation.
    Article Snippet: The human lymphotoxin receptor (LT R), a member of the tumor necrosis factor (TNF) receptor superfamily, is essential for not only the development and organization of secondary lymphoid tissues, but also for chemokine release.. Even though LT R was shown to recruit TNF-receptor-associated factor (TRAF) 2, 3, and 5, and to induce cell apoptosis or NFB activation, however, the downstream signaling leading to chemokine expression is not illustrated yet.. In this study, we find that overexpression of LT R in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release.

    Article Title: Norepinephrine Increases IκBα Expression in Astrocytes
    Article Snippet: .. An equal volume of luciferase substrate (Steady Glo reagent, Promega) was added to all samples, and the luminescence was measured in a microplate luminometer (Rosys-Anthos). .. Preparation of Cytosolic and Nuclear Fractions—Cells were washed in cold PBS, pelleted, and resuspended in hypotonic buffer (10 mM HEPES, pH 7.6, 1 mM EDTA, 10 mM KCl, 1 mM dithiothreitol) and protease inhibitors (Sigma).

    Article Title: Inhibitory and Stimulatory Effects of Lactacystin on Expression of Nitric Oxide Synthase Type 2 in Brain Glial Cells
    Article Snippet: .. An equal volume of luciferase substrate (Steady Glo reagent, Promega) was added to all samples, and the luminescence was measured in a microplate luminometer (Rosys-Anthos, Austria). ..

    Incubation:

    Article Title: Lymphotoxin beta receptor induces interleukin 8 gene expression via NF-kappaB and AP-1 activation.
    Article Snippet: The human lymphotoxin receptor (LT R), a member of the tumor necrosis factor (TNF) receptor superfamily, is essential for not only the development and organization of secondary lymphoid tissues, but also for chemokine release.. Even though LT R was shown to recruit TNF-receptor-associated factor (TRAF) 2, 3, and 5, and to induce cell apoptosis or NFB activation, however, the downstream signaling leading to chemokine expression is not illustrated yet.. In this study, we find that overexpression of LT R in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release.



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